Clotrimazole’s medicinal effects (Lotrimin) Clotrimazole functions by penetrating the fungus’ cytoplasmic membrane. Clotrimazole inhibits the demethylation of 14 alpha lanosterol, preventing the production of ergosterol. Also, clotrimazole is an antifungal medication and decreases the membrane’s permeability in fungi. This exercise explains clotrimazole’s indications