SCI260 – Introduction to Biochemistry
Lesson #9
(1) Which of the double-stranded DNA sequences below will require more heat to
separate the two strands (select Option A or Option B)? (2) Why is more heat is required
to separate the strands?
Option A
5’-GGACTCCCGGGTGAG-3’
3’-CCTGAGGGCCCACTC-5’
Option B
5’-AACTGTTTAAAGACA-3’
3’-TTGACAAATTTCTGT-5’
(3) What will be the mRNA sequence that is produced during the transcription of the
DNA sequence below? Hint: As we discussed in class, there is a slight difference in the
nucleotides used in DNA vs RNA.
5’-ACGTCAGGTTTCCGT-3’
(4) Based on what was discussed in Lecture 9, name four (4) enzymes/proteins that play
a role in human DNA replication, and briefly describe the specific role each plays during
human DNA replication.
You may use the internet and/or your textbook to assist you. Each answer is worth 25
points (be sure to answer ALL four questions) giving you a maximum total of 100 points
for this assignment. Please DO NOT copy your answers directly from the textbook or the
internet.
